SYK Knockout cell line (HCT 116)

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location： Home > Products > KO Cell Lines > SYK Knockout cell line (HCT 116)

EZ-editor™ Gene-editing Series Products

   CRISPR-iScreen™ Library

CRISPR-iScreen™ Library Plasmid

CRISPR-iScreen™ Library Virus

CRISPR-iScreen™ Cell Pool

   gRNA Plasmid Bank

   Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

   Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

   Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

   Lentivirus

gene KO/PM cell lines
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

SYK Knockout cell line (HCT 116) Order

SYK Knockout cell line (HCT 116)
Catalog#
YKO-HS2615
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SYK Knockout cell line (HCT 116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SYK Knockout cell line (HCT 116)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
SYK
Gene ID
6850
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Accession
CVCL_D8WQ(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript SYK-204)
Validation: PCR+Sanger Sequencing
SYK Knockout cell line (HCT 116) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116		 STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SYK gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SYK gene knockout SYK Knockout cell line (HCT 116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SYK Knockout cell line (HCT 116)
SYK Knockout cell line (HCT 116)
Catalog#
YKO-HS2615
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SYK Knockout cell line (THP-1)

    SYK Knockout cell line (THP-1)
      inquiry   More details

    Organism:
    Gene Name: SYK
    Gene ID: 6850
    Size: 1*10^6
    Catalog#: YKO-HT0054
    YKO-HT0054()
  • Syk Knockout cell line (Raw 264.7)

    Syk Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism:
    Gene Name: Syk
    Gene ID: 20963
    Size: 1*10^6
    Catalog#: YKO-MT0020
    YKO-MT0020()
  • Syk Knockout cell line (BV2)

    Syk Knockout cell line (BV2)
      inquiry   More details

    Organism: Mouse
    Gene Name: Syk
    Gene ID: 20963
    Size: 1*10^6
    Catalog#: YKO-M202
    YKO-M202(BV2)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SYK Knockout cell line (THP-1)

    SYK Knockout cell line (THP-1)

    Organism:
    Gene Name: SYK
    Gene ID: 6850
    Size: 1*10^6
    Catalog#: YKO-HT0054
      inquiry   More details
  • Syk Knockout cell line (Raw 264.7)

    Syk Knockout cell line (Raw 264.7)

    Organism:
    Gene Name: Syk
    Gene ID: 20963
    Size: 1*10^6
    Catalog#: YKO-MT0020
      inquiry   More details
  • Syk Knockout cell line (BV2)

    Syk Knockout cell line (BV2)

    Organism: Mouse
    Gene Name: Syk
    Gene ID: 20963
    Size: 1*10^6
    Catalog#: YKO-M202
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
Contact us